B1155

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B1155

Sigma

 

bisBenzimide H 33258

powder, ≥98% (HPLC and TLC), cell culture tested

Synonym:BBIH, HOE 33258, Hoechst 33258, 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride
CAS Number:23491-45-4
Linear Formula:C25H24N6O · 3HCl
Molecular Weight:533.88
Beilstein Registry Number:4088183
EC Number:245-690-6
MDL number:MFCD00012679
PubChem Substance ID:24891565

Description

Biochem/physiol ActionsMembrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.
ApplicationUseful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Properties

assay≥98% (HPLC and TLC)
formpowder
solubilityH2O: 10 mg/mL
suitabilitycell culture tested
 passes application test for fluorescence
storage temp.−20°C

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Gloves
Hazard CodesXn
Risk Statements22-36/38
Safety Statements26-36
WGK Germany3
RTECSSM1140500

References

referenceLatt, S.A. and Stetten, G. J. Histochem. Cytochem. 24, 24, (1976)